Pengaruh Pati Jagung dan Ubi Kayu Termodifikasi Enzim Pullulanase terhadap Kadar Glukosa Darah Tikus Wistar (Rattus norvegicus)
Effects of Pullulanase-Modified Maize and Cassava Starches on Blood Glucose Levels in Wistar Rats (Rattus norvegicus)
DOI:
https://doi.org/10.65046/latuo.v1i1.79Keywords:
Amylose, blood glucose, cassava starch, maize starch, pullulanase, Wistar ratsAbstract
Starch is a major dietary carbohydrate source, but postprandial glucose responses are influenced by starch structure and digestibility. Although pullulanase-mediated debranching has been widely investigated, in vivo evidence on how starch source and pullulanase modification duration affect blood glucose responses remains limited. This study aimed to analyze blood glucose responses in Wistar rats following the administration of maize and cassava starches modified with pullulanase for 12 and 24 hours. An experimental study was conducted using 24 male Wistar rats randomly assigned to six groups: control maize starch, 12- and 24-hour pullulanase-modified maize starch, control cassava starch, and 12- and 24-hour pullulanase-modified cassava starch. Starch was administered orally at a dose of 6.75 g/kg body weight, and blood glucose levels were measured at 0, 30, 60, 90, and 120 minutes. Amylose content was analyzed as supporting data. The results showed that control maize starch (JT₀) produced the highest blood glucose response, reaching 188.95 mg/dL at 60 minutes, whereas 12-hour pullulanase-modified cassava starch (KT₁) produced the lowest response, reaching 110.50 mg/dL. KT₁ also showed an increase in amylose content from 30.85% to 42.45%. These findings provide in vivo evidence that starch source and pullulanase modification conditions are associated with differences in blood glucose responses and indicate that longer modification does not necessarily result in a lower glucose responseReferences
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